AffiAB® Anti-Synaptophysin Antibody
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P08247 Human; SwissProt: Q62277 Mouse; SwissProt: P07825 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide (KLH-coupled) within human Synaptophysin aa 220-280.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:2000-1:10, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 34 kDa
Subcellular location
Cytoplasmic vesicle, Membrane, Synapse, Synaptosome.
Positive control
Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44.