![AffiAB® Anti-STAT1 alpha Antibody [SD20-75]](/web/image/product.product/88926/image_1024/%5BAFG-HB-3694%5D%20AffiAB%C2%AE%20Anti-STAT1%20alpha%20Antibody%20%5BSD20-75%5D%20?unique=a48b62f)
AffiAB® Anti-STAT1 alpha Antibody [SD20-75]
STAT1 is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to IFN-γ stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element; in response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon-Stimulated Response Element) promoter element. [12] In either case, binding of the promoter element leads to an increased expression of ISG (Interferon-Stimulated Genes) .
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P42224 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SD20-75
KO/KD
Knockdown validated
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human STAT1 alpha aa 1-50 / 750.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation, JAK-STAT signaling pathway
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
87 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
Hela cell lysate, 293 cell lysate, Hela, MCF-7, SKOV-3, human cervical tissue.