AffiAB® Anti-SREBP1 Antibody
This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1) , which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P36956 Human; SwissProt: Q9WTN3 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human SREBP1 from aa25-aa55.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:100; IHC-P: 1:100-1:500
Molecular Weight
122 kDa
Subcellular location
Endoplasmic reticulum membrane, Golgi apparatus membrane, COPII-coated vesicle membrane; Nucleus.
Positive control
HepG2 cell lysate, mouse heart tissue lysate, mouse liver tissue lysate, 293 cell lysate, Jurkat cell lysate, A549 cell lysate, Hela, human lung tissue, human placenta tissue.