AffiAB® Anti-SPATA13 Antibody
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q96N96 Human; SwissProt: Q5DU57 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P, FC
Predicted species reactivity
Mouse
Immunogen
Recombinant protein within Human SPATA13 aa 423-572 / 652.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/ml.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:50-1:200
; FC: 1:50-1:100
Molecular Weight
Predicted band size: 75 kDa
Subcellular location
Cell membrane, Cell projection, Cytoplasm, Membrane.
Positive control
Rat kidney tissue lysates, rat testis tissue, human colon cancer tissue, human kidney tissue, SHSY5Y.