AffiAB® Anti-SP1 Antibody [JF0950]
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) , may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P08047 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JF0950
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human SP1 aa 544-588 / 785.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Huntington's disease
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200
Molecular Weight
81 kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
Jurkat cell lysate, Hela cell lysate, Hela, MCF-7, SW480, human breast carcinoma tissue, human colon carcinoma tissue, human kidney tissue.