AffiAB® Anti-Sodium Potassium ATPase Antibody [ST0533]

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CAT# AFG-HB-3642
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-Sodium Potassium ATPase Antibody [ST0533]

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P05023 Human; SwissProt: P05026 Human; SwissProt: Q13733 Human; SwissProt: P14094 Mouse; SwissProt: Q8VDN2 Mouse; SwissProt: Q9WV27 Mouse; SwissProt: P06685 Rat; SwissProt: P07340 Rat; SwissProt: Q64541 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

ST0533

KO/KD

Knockdown validated

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human ATP1A1 aa 39-83 / 1023.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

cAMP signaling pathway, Neuroscience, cGMP-PKG signaling pathway

Recommended dilutions

WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:500; FC: 1:50-1:100

Molecular Weight

Predicted band size: 113 kDa

Subcellular location

Cell membrane, Melanosome.

Positive control

A549 cell lysates, Hela, MCF-7, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, rat kidney tissue.