AffiAB® Anti-Sodium Potassium ATPase Antibody [ST0533]
The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P05023 Human; SwissProt: P05026 Human; SwissProt: Q13733 Human; SwissProt: P14094 Mouse; SwissProt: Q8VDN2 Mouse; SwissProt: Q9WV27 Mouse; SwissProt: P06685 Rat; SwissProt: P07340 Rat; SwissProt: Q64541 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
ST0533
KO/KD
Knockdown validated
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human ATP1A1 aa 39-83 / 1023.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
cAMP signaling pathway, Neuroscience, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 113 kDa
Subcellular location
Cell membrane, Melanosome.
Positive control
A549 cell lysates, Hela, MCF-7, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, rat kidney tissue.