![AffiAB® Anti-SMYD3 Antibody [JM73-63]](/web/image/product.product/88863/image_1024/%5BAFG-HB-3627%5D%20AffiAB%C2%AE%20Anti-SMYD3%20Antibody%20%5BJM73-63%5D%20?unique=a48b62f)
AffiAB® Anti-SMYD3 Antibody [JM73-63]
Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9H7B4 Human; SwissProt: Q9CWR2 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM73-63
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human SMYD3 aa 251-300 / 428.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 49 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
Rat kidney tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, PC-12 cell lysate, NIH-3T3 cell lysate, HepG2, Hela cell lysate, LOVO, Hela, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue.