AffiAB® Anti-SMG5 Antibody
Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A) , thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9UPR3 Human; SwissProt: Q6ZPY2 Mouse; Entrez Gene: 681012 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human SMG5 aa 800-1, 000.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000
; FC: 1:500-1:1, 000
Molecular Weight
114 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
Jurkat cell lysate, Hela cell lysate, 293 cell lysate, THP-1 cell lysate, mouse testis tissue lysates, rat testis tissue lysates, Hela.