AffiAB® Anti-Smad2 Antibody
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad) , have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad proteins.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q15796 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human Smad2.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
衰老
Recommended dilutions
IF-Cell: 1:50-1:100; IHC-P:1:50-1:200
Molecular Weight
N/A
Subcellular location
Cytoplasm, Nucleus
Positive control
SW480, SK-BR-3, HepG2, mouse placenta tissue, human liver cancer tissue, mouse heart tissue, mouse skeletal muscle tissue