AffiAB® Anti-SIRT1 Antibody
SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q96EB6 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human SIRT1 aa 698-747 / 747.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
衰老, AMPK signaling pathway
Recommended dilutions
WB: 1:1, 000-1:2, 000
; IHC-P: 1:200
; FC: 1:100-1:200
Molecular Weight
Predicted band size: 82 kDa
Subcellular location
Nucleus, cytoplasm, Mitochondrion.
Positive control
Hela cell lysate, Jurkat cell lysate, F9 cell lysate, Hela, human colon carcinoma tissue, human lung carcinoma tissue, mouse liver tissue, mouse testis tissue.