AffiAB® Anti-SHP2 Antibody [SN07-32]
The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs) . Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP) , which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp) , has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q06124 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SN07-32
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human SHP2 aa 494-593 / 593.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
RAS signaling pathway, Immunology & Inflammation, JAK-STAT signaling pathway
Recommended dilutions
WB: 1:500-1:1000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 68 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
Hela cell lysate, 293 cell lysate, Hela, MCF-7, NIH/3T3, human kidney tissue.