AffiAB® Anti-sfGFP Antibody
Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa) : this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
N/A
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB
Predicted species reactivity
N/A
Immunogen
Synthetic peptide immune sequence is RDHMVLHEYVNAAGITC.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000
Molecular Weight
N/A
Subcellular location
N/A
Positive control
GFP11-tagged fusion protein.