![AffiAB® Anti-SF3B1 Antibody [JB40-32]](/web/image/product.product/88793/image_1024/%5BAFG-HB-3554%5D%20AffiAB%C2%AE%20Anti-SF3B1%20Antibody%20%5BJB40-32%5D%20?unique=a48b62f)
AffiAB® Anti-SF3B1 Antibody [JB40-32]
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O75533 Human; SwissProt: Q99NB9 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JB40-32
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, IF-Tissue, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human SF3B1 aa 1-105 / 1, 304.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IHC-P: 1:50
Molecular Weight
Predicted band size: 146 kDa
Subcellular location
Nucleus.
Positive control
Mouse thymus tissue lysate, Hela cell lysate, rat brain tissue, human colon carcinoma tissue, human skin tissue, human kidney tissue, mouse small intestine tissue.