![AffiAB® Anti-SF3A3 Antibody [JE64-15]](/web/image/product.product/88792/image_1024/%5BAFG-HB-3553%5D%20AffiAB%C2%AE%20Anti-SF3A3%20Antibody%20%5BJE64-15%5D%20?unique=b83e256)
AffiAB® Anti-SF3A3 Antibody [JE64-15]
This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q12874 Human; SwissProt: Q9D554 Mouse; Entrez Gene: 313583 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE64-15
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human SF3A3 aa 1-50/501.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:400
Molecular Weight
Predicted band size: 59 kDa
Subcellular location
Nucleus speckle, Nucleus.
Positive control
MCF-7 cell lysate, 293T cell lysate, Hela cell lysate, rat testis tissue lysates, rat brain tissue lysates, rat lung tissue lysate, mouse cerebellum tissue lysate, mouse stomach tissue lysate, rat kidney tissue, human testis tissue, human colon carcinoma tissue, human prostate tissue, mouse small intestine tissue.