AffiAB® Anti-SCA-1 Antibody
Sca-1 stands for "Stem cells antigen-1" (official gene symbol: Ly6a) . It consist of 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein (GPI-AP) of the LY6 gene family. It is the common biological marker used to identify hematopoitic stem cell (HSC) along with other markers. Sca-1 has a regenerative role in cardiac repair: Host cells with specific Sca-1+CD31− markers arise upon myocardial infarction, with evidence of expression of Sca-1 protein. Sca-1 plays a role in hematopoietic progenitor/stem cell lineage fate and c-kit expression. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P05533 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Mouse
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Mouse SCA-1 aa 51-94.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000
Molecular Weight
Predicted band size: 14 kDa
Subcellular location
Cell membrane
Positive control
Mouse kidney tissue lysate, NIH/3T3 cell lysate.