AffiAB® Anti-S100 alpha 6 Antibody [JF0976]
Calcyclin, also known as Prolactin receptor-associated protein (PRA) , growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6) or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family whose expression is upregulated in proliferating and differentiating cells. Calcyclin is inducible by growth factors and overexpressed in acute myeloid leukemias. It is expressed in a cell-specific manner in subpopulations of neurons and astrocytes and in epithelial cells and fibroblasts. Calcyclin is a specific target of S-100B protein in vivo. The binding of Calcyclin to S-100B is stabilized by S-100B-bound calcium and zinc. Calcyclin associates with both Annexin XI and CacyBP (calcyclin-binding protein) . It functions to activate several processes along the calcium signal transduction pathway including the regulation of cell growth, proliferation, secretion and exocytosis.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P06703 Human; SwissProt: P14069 Mouse; SwissProt: P05964 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JF0976
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
Rat
Immunogen
Recombinant full length protein of Human S100 alpha 6 aa 1-90 / 90.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:5, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
10 kDa
Subcellular location
Nucleus envelope, Cytoplasm, Cell membrane.
Positive control
A431 cell lysate, mouse lung tissue lysate, Hela, A431, SH-SY5Y, human kidney tissue, human stomach carcinoma tissue, human colon carcinoma tissue, mouse brain tissue, mouse kidney tissue.