AffiAB® Anti-RUNX3 Antibody [A7B7]

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CAT# AFG-HB-3469
Size: 100 μL

465.00 465.0 USD 465.00

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AffiAB® Anti-RUNX3 Antibody [A7B7]

Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. In melanocytic cells RUNX3 gene expression may be regulated by MITF. Most of this literature used RUNX3 promoter methylation status in various cancers as a proxy for its expression. However, quite many genes are known to be methylated in tumor cell genomes, and the majority of these genes are not expressed in the normal tissue of origin of these cancers.

Antibody type

Mouse monoclonal Antibody

Uniprot ID

SwissProt: Q13761 Human; SwissProt: Q91ZK1 Rat

Recombinant

NO

Conjugation

Non-conjugated

Host

Mouse

Isotype

IgG1

Clone

A7B7

KO/KD

N/A

Species reactivity

Human, Rat

Tested applications

WB, IHC-P

Predicted species reactivity

N/A

Immunogen

Recombinant protein within human RUNX3 aa 151-350/415.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

PBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500-1:2, 000 ; IHC-P: 1:200-1:600

Molecular Weight

Predicted band size: 44 kDa

Subcellular location

Cytoplasm, Nucleus.

Positive control

SW480 cell lysates, Raji cell lysates, rat large intestine tissue, human tonsils tissue, human colon tissue.