AffiAB® Anti-Rubisco activase Antibody
Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize, Chlamydomonas) . Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P10896 Arabidopsis thaliana
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Zea mays, Spinacia oleracea, Morus alba
Tested applications
WB, IHC-P, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Arabidopsis thaliana Rubisco activase aa 190-233.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:10, 000-1:50, 000; IHC-P: 1:50-1:200; IF-Tissue: 1:50-1:200
Molecular Weight
N/A
Subcellular location
Plastid
Positive control
Mulberry leaf, rice, A. thaliana, tabacum.