AffiAB® Anti-RSPO2 Antibody
R-spondin 2 also known as roof plate-specific spondin-2 is a secreted protein that in humans that is encoded by the RSPO2 gene. R-spondin 2 synergizes with canonical WNT to activate beta-catenin. RSPO2 has been proposed to regulate craniofacial patterning and morphogenesis within pharyngeal arch 1 through ectoderm-mesenchyme signaling via the endothelin-Dlx5/6 pathway. In dogs, a variant on the Rspo2 gene is associated moustache and eyebrow thickness. In humans, recessive mutations in RSPO2 abrogate limb and lung development. Bruno Reversade and colleagues have reported in 2018 that loss of RSPO2 results in a syndrome of Tetra-amelia with lung agenesis.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q6UXX9 Human; SwissProt: Q8BFU0 Mouse; Entrez Gene: 500863 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human RSPO2 aa 51-100 / 243.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
5.17 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000
; IHC-P: 1:500-1:1, 000
Molecular Weight
Predicted band size: 28 kDa
Subcellular location
Secreted.
Positive control
293 cell lysate, human lung tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human kidney tissue, mouse small intestine tissue.