AffiAB® Anti-RRM1 Antibody [JB09-43]
This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1) , with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P23921 Human; SwissProt: P07742 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JB09-43
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, FC, IP
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human RRM1 aa 651-792 / 792.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:100
; FC: 1:50-1:100
; IP:1:10-1:50
Molecular Weight
Predicted band size: 90 kDa
Subcellular location
Cytoplasm.
Positive control
Mouse thymus tissue lysate, A431 cell lysate, human colon tissue, A431.