AffiAB® Anti-RPS20 Antibody [SC65-08]
Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5’ and 3’ minor domains of 16S ribosomal RNA (rRNA) . Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P60866 Human; SwissProt: P60867 Mouse; SwissProt: P60868 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SC65-08
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
Rat
Immunogen
Synthetic peptide within Human RPS20 aa 36-80 / 119.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 13 kDa
Subcellular location
Cytoplasm.
Positive control
Hela cell lysate, HUVEC cell lysate, Hela, B16F1, human colon carcinoma tissue, human prostate carcinoma tissue, mouse hippocampus tissue, mouse brain tissue.