AffiAB® Anti-ROCK1 Antibody
ROCK1 is a protein serine/threonine kinase also known as rho-associated, coiled-coil-containing protein kinase 1. ROCK1 has a ubiquitous tissue distribution, but subcellularly it is thought to colocalize with the centrosomes. This is consistent with its function as a key modulator of cell motility, tumor cell invasion, and actin cytoskeleton organization. ROCK1 has a diverse range of functions in the body. It is a key regulator of actin-myosin contraction, stability, and cell polarity. These contribute to many progresses such as regulation of morphology, gene transcription, proliferation, differentiation, apoptosis and oncogenic transformation. Other functions involve smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility. These functions are activated by phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q13464 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within residues of ROCK1 aa 1, 305-1, 354 / 1, 354.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
cAMP signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:1, 000; IHC-P: 1:200; IF-Cell: 1:50; FC: 1:100-1:200
Molecular Weight
Predicted band size: 158 kDa
Subcellular location
Cytoplasm, cell membrane, Nucleus, Cell projection, Cytoskeleton, Golgi apparatus, Membrane.
Positive control
Hela cell lysate, NIH/3T3 cell lysate, Jurkat cell lysate, human colon cancer tissue, HepG2.