![AffiAB® Anti-RNF40 Antibody [JE46-30]](/web/image/product.product/88673/image_1024/%5BAFG-HB-3430%5D%20AffiAB%C2%AE%20Anti-RNF40%20Antibody%20%5BJE46-30%5D%20?unique=d31c23b)
AffiAB® Anti-RNF40 Antibody [JE46-30]
E3 ubiquitin-protein ligase BRE1B is an enzyme that in humans is encoded by the RNF40 gene. The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O75150 Human; SwissProt: Q3U319 Mouse; SwissProt: Q8CJB9 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE46-30
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IP, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human RNF40 aa 211-451 / 1, 001.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/ml.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:1, 000-1:2, 000
; IP:1:10-1:50
; IHC-P:1:50-1:400
Molecular Weight
Predicted band size: 114 kDa
Subcellular location
Nucleus.
Positive control
Mouse testis tissue lysate, rat brain tissue lysate, A431 cell lysate, SH-SY-5Y cell lysate, SiHa cell lysate, 293 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate, human colon tissue, mouse brain tissue.