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AffiAB® Anti-RhoGAP Antibody [JE53-53]
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of RASA1/p120GAP. Impaired autophagic degradation of lncRNA ARHGAP5-AS1 in chemoresistant cancer cells promoted chemoresistance. It can activate the transcription of ARHGAP5 in the nucleus and stimulate m6A modification of ARHGAP5 mRNA to stabilize ARHGAP5 mRNA in the cytoplasm by recruiting METTL3. Therefore, targeting ARHGAP5-AS1/ARHGAP5 axis might be a promising strategy to overcome chemoresistance in gastric cancer.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q13017 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE53-53
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human ARHGAP5 aa 1, 130-1, 320 / 1, 502.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:500-1:2, 000
; FC:1:50-1:100
Molecular Weight
172 kDa
Subcellular location
Cell membrane, cytoplasm.
Positive control
SiHa cell lysate, HepG2 cell lysate, SiHa.