AffiAB® Anti-Rho A+B+C Antibody [SR38-00]
Transforming protein RhoA, also known as Ras homolog family member A (RhoA) , is a small GTPase protein in the Rho family of GTPases that in humans is encoded by the RHOA gene. RhoA, and the other Rho GTPases, are part of a larger family of related proteins known as the Ras superfamily, a family of proteins involved in the regulation and timing of cell division. RhoA specifically is regarded as a prominent regulatory factor in other functions such as the regulation of cytoskeletal dynamics, transcription, cell cycle progression and cell transformation. Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. RHOB is a member of the Rho GTP-binding protein family. RhoC (Ras homolog gene family, member C) is a small (~21 kDa) signaling G protein (more specifically a GTPase) , and is a member of the Rac subfamily of the family Rho family of GTPases. It is encoded by the gene RHOC.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P08134 Human; SwissProt: P61586 Human; SwissProt: P62745 Human; SwissProt: P62746 Mouse; SwissProt: Q62159 Mouse; SwissProt: Q9QUI0 Mouse; SwissProt: P61589 Rat; SwissProt: P62747 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR38-00
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
Rat
Immunogen
Recombinant protein within Human RhoC aa aa 1-193 / 193.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IF-Cell: 1:50; IF-Tissue: 1:50; IHC-P: 1:50; FC: 1:50
Molecular Weight
Predicted band size: 22 kDa
Subcellular location
Cytoplasm, Nucleus, Cell membrane, Cleavage furrow.
Positive control
HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, AGS, HepG2, NIH/3T3, human stomach carcinoma tissue, mouse colon tissue, mouse stomach tissue.