AffiAB® Anti-RHEB Antibody [JG37-12]
H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein) , also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q15382 Human; SwissProt: Q921J2 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JG37-12
KO/KD
Knockout validated
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human RHEB aa 21-108 / 184.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
20 kDa
Subcellular location
Cytoplasm. Golgi apparatus. Endoplasmic reticulum.
Positive control
Wild-type Raw264.7 whole cell lysate, RHEB knockdown Raw264.7 whole cell lysate, mouse placenta tissue lysates, A431, SH-SY5Y, human liver tissue, human colon tissue, human fetal skeletal muscle tissue, Hela.