AffiAB® Anti-RHEB Antibody [JG37-12]

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CAT# AFG-HB-3409
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-RHEB Antibody [JG37-12]

H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein) , also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: Q15382 Human; SwissProt: Q921J2 Mouse

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JG37-12

KO/KD

Knockout validated

Species reactivity

Human, Mouse

Tested applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Predicted species reactivity

N/A

Immunogen

Recombinant protein within Human RHEB aa 21-108 / 184.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

Molecular Weight

20 kDa

Subcellular location

Cytoplasm. Golgi apparatus. Endoplasmic reticulum.

Positive control

Wild-type Raw264.7 whole cell lysate, RHEB knockdown Raw264.7 whole cell lysate, mouse placenta tissue lysates, A431, SH-SY5Y, human liver tissue, human colon tissue, human fetal skeletal muscle tissue, Hela.