AffiAB® Anti-RBBP5 Antibody
In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q15291 Human; SwissProt: Q8BX09 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human RBBP5 aa 1-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2.1 mg/ml
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
Molecular Weight
Predicted band size: 59 kDa
Subcellular location
Nucleus.
Positive control
293 cell lysate, MCF-7 cell lysate, human lung carcinoma tissue, human colon tissue.