![AffiAB® Anti-Raf1 Antibody [JJ086-06]](/web/image/product.product/88607/image_1024/%5BAFG-HB-3356%5D%20AffiAB%C2%AE%20Anti-Raf1%20Antibody%20%5BJJ086-06%5D%20?unique=a48b62f)
AffiAB® Anti-Raf1 Antibody [JJ086-06]
RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. The c-Raf protein is part of the ERK1/2 pathway as a MAP kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P04049 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JJ086-06
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human Raf1 aa 1-50/648.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
VEGF signaling pathway, PI3K-AKT, 衰老, cAMP signaling pathway, ErbB signaling pathway, RAS signaling pathway, Immunology & Inflammation, Autophagy Alzheimer disease, MAPK signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200
Molecular Weight
Predicted band size: 73 kDa
Subcellular location
Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
Positive control
HepG2 cell lysates, Hela, MCF-7.