![AffiAB® Anti-RAD18 Antibody [SN66-01]](/web/image/product.product/88600/image_1024/%5BAFG-HB-3348%5D%20AffiAB%C2%AE%20Anti-RAD18%20Antibody%20%5BSN66-01%5D%20?unique=a48b62f)
AffiAB® Anti-RAD18 Antibody [SN66-01]
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9NS91 Human; SwissProt: Q9QXK2 Mouse; Unigene: 22793 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SN66-01
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human RAD18 aa 50-99 / 495.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 56 kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
NIH/3T3 cell lysate, MCF-7 cell lysate, Hela, NIH/3T3, 293, rat testis tissue.