AffiAB® Anti-Rab4A Antibody [SR27-08]
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P20338 Human; SwissProt: P56371 Mouse; SwissProt: P05714 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR27-08
KO/KD
N/A
Species reactivity
Human, Rat, Monkey
Tested applications
WB, IF-Cell, IF-Tissue, IP
Predicted species reactivity
Mouse
Immunogen
Synthetic peptide within Human Rab4A aa 169-218 / 218.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Neuroscience
Recommended dilutions
WB: 1:500; IF-Cell: 1:50; IF-Tissue: 1:50; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 24 kDa
Subcellular location
Cytoplasm, Membrane
Positive control
MCF-7 cell lysate, 293T cell lysate, COS-1 cell lysate, PC-12.