AffiAB® Anti-Rab11-FIP3 Antibody
Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O75154 Human; SwissProt: Q8CHD8 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within mouse FIP3 aa 543-592 / 1, 047.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:500-1:2, 000; IHC-P:1:50-1:200
Molecular Weight
Predicted band size 82/119 kDa.
Subcellular location
Recycling endosome membrane, centrosome, Cleavage furrow, Midbody.
Positive control
MCF-7 cell lysates, mouse liver tissue, mouse kindey tissue.