![AffiAB® Anti-Pumilio 1 Antibody [JG37-75]](/web/image/product.product/77775/image_1024/%5BAFG-HB-3322%5D%20AffiAB%C2%AE%20Anti-Pumilio%201%20Antibody%20%5BJG37-75%5D%20?unique=a48b62f)
AffiAB® Anti-Pumilio 1 Antibody [JG37-75]
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE) , 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) . Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q14671 Human; SwissProt: Q80U78 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JG37-75
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, FC, IP, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human Pumilio 1 aa 79-223 .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: 1:10-1:50
Molecular Weight
Predicted band size: 126 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
SiHa cell lysate, 293T, SiHa, human colon tissue, human appendix tissue, mouse kidney tissue, mouse small intestine tissue.