![AffiAB® Anti-PTBP1 Antibody [9A1]](/web/image/product.product/77762/image_1024/%5BAFG-HB-3306%5D%20AffiAB%C2%AE%20Anti-PTBP1%20Antibody%20%5B9A1%5D%20?unique=8544d55)
AffiAB® Anti-PTBP1 Antibody [9A1]
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P26599 Human; SwissProt: Q00438 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2a
Clone
9A1
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human PTBP1 aa 320-550.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:5, 000-1:10, 000; IHC-P: 1:50-1:100; IF-Cell: 1:50-1:100; FC: 1:50-1:100
Molecular Weight
Predicted band size: 57 kDa
Subcellular location
Nucleus.
Positive control
Jurkat cell lysate, A431 cell lysate, K562 cell lysate, A549, 293T, SW480, human kidney tissue, human tonsil tissue, rat testis tissue, human colon cancer tissue, K562.