![AffiAB® Anti-PRPF4 Antibody [JE65-37]](/web/image/product.product/77722/image_1024/%5BAFG-HB-3265%5D%20AffiAB%C2%AE%20Anti-PRPF4%20Antibody%20%5BJE65-37%5D%20?unique=b83e256)
AffiAB® Anti-PRPF4 Antibody [JE65-37]
The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O43172 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE65-37
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human PRPF4 aa 1-50/522.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IF-Cell: 1:50-1:200; IHC-P: 1:100-1:400
Molecular Weight
Predicted band size: 58 kDa
Subcellular location
Nucleus, Nucleus speckle.
Positive control
HT-29 cell lysate, SW480 cell lysate, MCF-7 cell lysate, SiHa, human cervical carcinoma tissue, human colon tissue, H1395.