AffiAB® Anti-Proteasome 20S LMP7 Antibody [JB54-32]

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CAT# AFG-HB-3259
Size: 100 μL

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AffiAB® Anti-Proteasome 20S LMP7 Antibody [JB54-32]

Proteasome subunit beta type-8 as known as 20S proteasome subunit beta-5i is a protein that in humans is encoded by the PSMB8 gene. This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, beta5i) that contributes to the complete assembly of 20S proteasome complex. In particular, proteasome subunit beta type-5, along with other beta subunits, assemble into two heptameric rings and subsequently a proteolytic chamber for substrate degradation. This protein contains "Chymotrypsin-like" activity and is capable of cleaving after large hydrophobic residues of peptide. The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. The constitutive subunit beta1, beta2, and beta 5 (systematic nomenclature) can be replaced by their inducible counterparts beta1i, 2i, and 5i when cells are under the treatment of interferon-γ. The resulting proteasome complex becomes the so-called immunoproteasome. An essential function of the modified proteasome complex, the immunoproteasome, is the processing of numerous MHC class-I restricted T cell epitopes.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P28062 Human; SwissProt: P28063 Mouse; SwissProt: P28064 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JB54-32

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Predicted species reactivity

N/A

Immunogen

Recombinant protein within Human Proteasome 20S LMP7 aa 160-276 / 276.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

Molecular Weight

Predicted band size: 30 kDa

Subcellular location

Nucleus. Cytoplasm.

Positive control

U937 cell lysate, A431 cell lysate, HUVEC, A431, mouse small intestine tissue, human kidney tissue, rat epididymis tissue, human liver tissue, Daudi.