AffiAB® Anti-Proteasome 20S C2 Antibody [JB89-30]

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CAT# AFG-HB-3257
Size: 100 μL

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AffiAB® Anti-Proteasome 20S C2 Antibody [JB89-30]

Proteasome subunit alpha type-1 is a protein that in humans is encoded by the PSMA1 gene. This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, and beta5i) that contributes to the complete assembly of 20S proteasome complex. Crystal structures of isolated 20S proteasome complex demonstrate that the two rings of beta subunits form a proteolytic chamber and maintain all their active sites of proteolysis within the chamber. Concomitantly, the rings of alpha subunits form the entrance of substrate entering the proteolytic chamber. In an inactivated 20S proteasome complex, the gate into the internal proteolytic chamber are guarded by N-terminal tails of specific alpha-subunit. The proteolytic capacity of 20S core particle (CP) can be activated when CP associates with one or two regulatory particles (RP) on one or both side of alpha rings. These regulatory particles include 19S proteasome complexes, 11S proteasome complex, etc. Following the CP-RP association, the confirmation of certain alpha subunits will change and consequently cause the opening of substrate entrance gate.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P25786 Human; SwissProt: Q9R1P4 Mouse; SwissProt: P18420 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JB89-30

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IHC-P, FC, IP

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human Proteasome 20S C2 aa 214-263 / 263.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

Parkinson’s disease, Huntington's disease, Amyotrophic lateral sclerosis, Alzheimer disease

Recommended dilutions

WB: 1:500-1:2, 000 ; IHC-P: 1:50-1:200 ; FC: 1:50-1:100 ; IP: Use at an assay dependent concentration.

Molecular Weight

Predicted band size: 30 kDa

Subcellular location

Cytoplasm. Nucleus.

Positive control

PC-3M cell lysate, K562 cell lysate, rat testis tissue lysate, mouse testis tissue lysate, mouse colon tissue lysate, human pancreas tissue, rat testis tissue, mouse cerebellum tissue, human liver cancer tissue, K562.