AffiAB® Anti-PPM1A Antibody
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P35813 Human; SwissProt: P49443 Mouse; SwissProt: P20650 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human PP2C alpha aa 42-330 / 382.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
MAPK signaling pathway
Recommended dilutions
WB: 1:500; IF-Cell: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
42/35 kDa, additional band 55 kDa.
Subcellular location
Cytoplasm, Membrane, Nucleus.
Positive control
Mouse kidney tissue lysate, rat skin tissue lysate, A431, A549, SH-SY5Y, human kidney tissue, human colon cancer tissue, mouse intestine tissue.