AffiAB® Anti-PLCG2 Antibody
Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate.There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR γ chain) , which bind to its C-terminal SH2 domain. PLC γ1 associates with Syk in B cells, but PLC γ2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC γ2. In addition, Btk can induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P16885 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human PLCG2 aa 1200-1265.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, Calcium signaling pathway, NF-KB signaling pathway, ErbB signaling pathway, RAS signaling pathway, Immunology & Inflammation, Phosphatidylinositol signaling
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:100-1:400; FC: 1:50-1:100
Molecular Weight
148 kDa
Subcellular location
Cytosol, Autolysosome.
Positive control
Raji cell lysates, AGS, human tonsil tissue, Daudi.