AffiAB® Anti-PKC epsilon Antibody [JA38-21]
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q02156 Human; SwissProt: P16054 Mouse; SwissProt: P09216 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JA38-21
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human PKC epsilon aa 561-610 / 737.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Type II diabetes mellitus, Immunology & Inflammation, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
84 kDa
Subcellular location
Cytoplasm.?Membrane.?Nucleus.
Positive control
MCF-7 cell lysate, Jurkat cell lysate, HT-29 cell lysate, 293T, Hela, rat brain tissue, human kidney tissue, mouse testis tissue, human pancreas tissue, SH-SY5Y.