![AffiAB® Anti-PIM1 Antibody [ST0513]](/web/image/product.product/77604/image_1024/%5BAFG-HB-3140%5D%20AffiAB%C2%AE%20Anti-PIM1%20Antibody%20%5BST0513%5D%20?unique=a48b62f)
AffiAB® Anti-PIM1 Antibody [ST0513]
Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P11309 Human; SwissProt: P06803 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
ST0513
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
Mouse
Immunogen
Synthetic peptide within human PIM1 aa 20-60.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation, JAK-STAT signaling pathway
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:400
Molecular Weight
Predicted band size: 36 kDa
Subcellular location
Cytoplasm, Nucleus, Cell membrane.
Positive control
BT-20 cell lysate, HepG2 cell lysate, SW480 cell lysate, BT-20, HepG2, SW480, human tonsil tissue, human colon carcinoma tissue, human spleen tissue, human breast carcinoma tissue, human breast tissue.