AffiAB® Anti-Phospho-P38 (Thr180 + Tyr182) Antibody
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q16539 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB
Predicted species reactivity
Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr180 and Y182 of human P38.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
VEGF signaling pathway, 衰老, Immunology & Inflammation, MAPK signaling pathway, Amyotrophic lateral sclerosis
Recommended dilutions
WB: 1:500-1:2, 000
Molecular Weight
41 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
293 cell lysate untreated with UV, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate untreated with UV, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate, Hela cell lysate treated with UV for 40 minutes, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes.