![AffiAB® Anti-Phospho-MEK1 (S218/S222) Antibody [ST0490]](/web/image/product.product/77532/image_1024/%5BAFG-HB-3062%5D%20AffiAB%C2%AE%20Anti-Phospho-MEK1%20%28S218-S222%29%20Antibody%20%5BST0490%5D%20?unique=a48b62f)
AffiAB® Anti-Phospho-MEK1 (S218/S222) Antibody [ST0490]
Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q02750 Human; SwissProt: P31938 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
ST0490
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of Human MEK1 aa 200-249 / 393.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, PI3K-AKT, 衰老, cAMP signaling pathway, ErbB signaling pathway, RAS signaling pathway, Immunology & Inflammation, Autophagy Alzheimer disease, MAPK signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 44 kDa
Subcellular location
Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Positive control
Hela cell lysate, A431 cell lysate, 293T cell lysate, Hela, HepG2, NIH/3T3, human tonsil tissue, human liver carcinoma tissue, human spleen tissue, human breast carcinoma tissue.