![AffiAB® Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09]](/web/image/product.product/77515/image_1024/%5BAFG-HB-3043%5D%20AffiAB%C2%AE%20Anti-Phospho-Histone%20H2A.X%20%28S139%29%20Antibody%20%5BSR33-09%5D%20?unique=d31c23b)
AffiAB® Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09]
Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139) , which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P16104 Human; SwissProt: P27661 Mouse; Unigene:2850 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR33-09
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:50-1:500
Molecular Weight
Predicted band size: 15 kDa
Subcellular location
Nucleus, Chromosome
Positive control
HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue, Hela cell lysate treated with UV.