![AffiAB® Anti-Phospho-EIF2S1 (S51) Antibody [SZ01-06]](/web/image/product.product/77501/image_1024/%5BAFG-HB-3029%5D%20AffiAB%C2%AE%20Anti-Phospho-EIF2S1%20%28S51%29%20Antibody%20%5BSZ01-06%5D%20?unique=a48b62f)
AffiAB® Anti-Phospho-EIF2S1 (S51) Antibody [SZ01-06]
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR) , endoplasmic reticulum stress (PERK/PEK) , amino acid deprivation (GCN2) , or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P05198 Human; SwissProt: Q6ZWX6 Mouse; SwissProt: P68101 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SZ01-06
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser51 of Human eIF-2a.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:500; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 36 kDa
Subcellular location
Stress granule, Cytoplasm.
Positive control
HUVEC cell lysate, Hela cell lysate, PC-3M cell lysate, THP-1 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue, human liver tissue, human pancreas tissue, mouse brain tissue, mouse placenta tissue, mouse pancreas tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human colon carcinoma tissue.