![AffiAB® Anti-Phospho-Creb (S133) Antibody [JB25-40]](/web/image/product.product/77495/image_1024/%5BAFG-HB-3023%5D%20AffiAB%C2%AE%20Anti-Phospho-Creb%20%28S133%29%20Antibody%20%5BJB25-40%5D%20?unique=a48b62f)
AffiAB® Anti-Phospho-Creb (S133) Antibody [JB25-40]
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE) , a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P16220 Human; SwissProt: Q01147 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JB25-40
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, IP, FC
Predicted species reactivity
Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Huntington's disease, Neuroscience, PI3K-AKT, Immunology & Inflammation, cAMP signaling pathway, AMPK signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:400; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 35 kDa
Subcellular location
Nucleus.
Positive control
NIH/3T3 cell lysates, THP-1 cell lysates, SH-SY5Y, HUVEC, human spleen tissue, mouse colon tissue, human colon carcinoma tissue, human lymph nodes tissue, mouse large intestine tissue.