AffiAB® Anti-Phospho-AKT2 (S474) Antibody
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P31751 Human; SwissProt: Q60823 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human AKT2 aa 450-481 (phospho S474) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:100-1:500
Molecular Weight
56 kDa
Subcellular location
Cell membrane, Nucleus, Early endosome, Cytoplasm.
Positive control
Hela treated with Calyculin A whole cell lysate, Hela treated with insulin whole cell lysate, human kidney tissue, mouse testis tissue.