AffiAB® Anti-PFAS Antibody
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O15067 Human; SwissProt: Q5SUR0 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human PURL aa 1, 133-1, 338 / 1, 338.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000
; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 145 kDa
Subcellular location
Cytoplasm.
Positive control
293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate, 293 cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human kidney tissue.