![AffiAB® Anti-pan Cytokeratin Antibody [PD00-15]](/web/image/product.product/77365/image_1024/%5BAFG-HB-2884%5D%20AffiAB%C2%AE%20Anti-pan%20Cytokeratin%20Antibody%20%5BPD00-15%5D%20?unique=b83e256)
AffiAB® Anti-pan Cytokeratin Antibody [PD00-15]
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium.
Antibody type
Recombinant Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q01546 Human; SwissProt: Q7Z794 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Mouse
Isotype
N/A
Clone
PD00-15
KO/KD
N/A
Species reactivity
Human
Tested applications
IHC-P, IF-Cell
Predicted species reactivity
N/A
Immunogen
Recognizes human cytokeratins 4, 5, 6, 8, 10, 13, and 18.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
IHC-P: 1:500-1:1, 000; IF-Cell: 1:50
Molecular Weight
N/A
Subcellular location
Cytoplasm.
Positive control
Human skin tissue, human breast tissue, human small intestine tissue, MCF-7.