AffiAB® Anti-P53 Antibody
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. P53 is found in increased amounts in a wide variety of transformed cells. P53 is frequently mutated or inactivated in about 60% of cancers.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P04637 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human P53.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Huntington's disease, PI3K-AKT, 衰老, p53 signaling pathway, Cancer, Ferroptosis, Apotosis, MAPK signaling pathway, Parkinson’s disease, Amyotrophic lateral sclerosis
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:100
Molecular Weight
Predicted band size: 53 kDa
Subcellular location
Cytoplasm, Nucleus, Cytoskeleton, Endoplasmic reticulum, Mitochondrion.
Positive control
293 cell lysate, A431.