AffiAB® Anti-p50 dynamitin Antibody
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD) . The subunit is commonly referred to as p50 or dynamitin. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that bind to two copies of p150-glued (DCTN1) and two copies of p24 (DCTN3) to form the dynactin shoulder domain.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q13561 Human; SwissProt: Q99KJ8 Mouse; SwissProt: Q6AYH5 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human p50 dynamitin aa 1-200.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Huntington's disease, Amyotrophic lateral sclerosis
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:200-1:1, 000; FC: 1:50-1:100
Molecular Weight
Predicted band size: 44 kDa.
Subcellular location
Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule.
Positive control
SHSY5Y cell lysate, mouse brain tissue lysate, PANC-1, rat cerebellum tissue, SHSY5Y.